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***How does it work?*** Imagine you have a mixture of different proteins, each with a unique pI. In isofocusing, these proteins are placed in a pH gradient within a gel or a capillary. When an electric field is applied, the proteins will migrate through the gradient until they reach the point where the pH equals their pI. At this point, they become electrically neutral and stop migrating, effectively becoming focused or concentrated at that specific location. This allows researchers to separate and analyze the different proteins present in the CSF sample. The beauty of isofocusing lies in its high resolution and ability to separate proteins that have very similar properties. This makes it an invaluable tool for detecting subtle changes in protein profiles, which can be indicative of various neurological disorders.
